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anti-human jaw1 antibody  (Operon Biotech)

 
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    Operon Biotech anti-human jaw1 antibody
    <t>Jaw1</t> increases the Ca 2+ influx depending on its own expression level. ( A , B , E , F , L ) Mean curves of relative Fluo-4 ( A , E , L ) or Rhod-2 ( B , F ) intensity upon 100 µM ATP stimulation measured using a plate reader. WT, Jaw1 KO #7, and Jaw1 KO #17 cells ( A , B ); Jaw1 KO, Jaw1 IE, and Δcoil IE cells ( E , F ); or Jaw1 KO, Jaw1 IE medium, and Jaw1 IE high cells ( L ). The closed triangles indicate the time point of 100 µM ATP solution supplementation. ( C , D , G , H , M ) AUC in ( A ), ( B ), ( E ), ( F ), and ( L ) are shown in ( C ), ( D ), ( G ), ( H ), and ( M ), respectively. n = 3. ( I ) Relative expression level of jaw1 mRNA to gapdh in WT and Jaw1 IE cells measured by RT-qPCR. N = 3. ( J ) Jaw1 KO and Jaw1 IE cells treated with 0.25 or 200 ng/mL of Dox and subjected to western blotting. Images used for western blots are shown in Supplementary Fig. online. ( K ) The relative protein expression level of Jaw1 with α-Tubulin in ( J ). n = 3. The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.
    Anti Human Jaw1 Antibody, supplied by Operon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-human jaw1 antibody/product/Operon Biotech
    Average 90 stars, based on 1 article reviews
    anti-human jaw1 antibody - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Jaw1/LRMP increases Ca 2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype"

    Article Title: Jaw1/LRMP increases Ca 2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-13620-4

    Jaw1 increases the Ca 2+ influx depending on its own expression level. ( A , B , E , F , L ) Mean curves of relative Fluo-4 ( A , E , L ) or Rhod-2 ( B , F ) intensity upon 100 µM ATP stimulation measured using a plate reader. WT, Jaw1 KO #7, and Jaw1 KO #17 cells ( A , B ); Jaw1 KO, Jaw1 IE, and Δcoil IE cells ( E , F ); or Jaw1 KO, Jaw1 IE medium, and Jaw1 IE high cells ( L ). The closed triangles indicate the time point of 100 µM ATP solution supplementation. ( C , D , G , H , M ) AUC in ( A ), ( B ), ( E ), ( F ), and ( L ) are shown in ( C ), ( D ), ( G ), ( H ), and ( M ), respectively. n = 3. ( I ) Relative expression level of jaw1 mRNA to gapdh in WT and Jaw1 IE cells measured by RT-qPCR. N = 3. ( J ) Jaw1 KO and Jaw1 IE cells treated with 0.25 or 200 ng/mL of Dox and subjected to western blotting. Images used for western blots are shown in Supplementary Fig. online. ( K ) The relative protein expression level of Jaw1 with α-Tubulin in ( J ). n = 3. The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.
    Figure Legend Snippet: Jaw1 increases the Ca 2+ influx depending on its own expression level. ( A , B , E , F , L ) Mean curves of relative Fluo-4 ( A , E , L ) or Rhod-2 ( B , F ) intensity upon 100 µM ATP stimulation measured using a plate reader. WT, Jaw1 KO #7, and Jaw1 KO #17 cells ( A , B ); Jaw1 KO, Jaw1 IE, and Δcoil IE cells ( E , F ); or Jaw1 KO, Jaw1 IE medium, and Jaw1 IE high cells ( L ). The closed triangles indicate the time point of 100 µM ATP solution supplementation. ( C , D , G , H , M ) AUC in ( A ), ( B ), ( E ), ( F ), and ( L ) are shown in ( C ), ( D ), ( G ), ( H ), and ( M ), respectively. n = 3. ( I ) Relative expression level of jaw1 mRNA to gapdh in WT and Jaw1 IE cells measured by RT-qPCR. N = 3. ( J ) Jaw1 KO and Jaw1 IE cells treated with 0.25 or 200 ng/mL of Dox and subjected to western blotting. Images used for western blots are shown in Supplementary Fig. online. ( K ) The relative protein expression level of Jaw1 with α-Tubulin in ( J ). n = 3. The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Jaw1 increases Ca 2+ influx by changing the Ca 2+ influx pattern. ( A , B , C ) Representative relative intensity traces upon 100 µM ATP stimulation: ( A ) single, ( B ) oscillation, and ( C ) steady reduction types. ( D , G ) Mean ( D ) or five representative ( G ) curves of the relative intensity in Jaw1 KO, Jaw1 IE, and Δcoil IE cells. The closed triangles indicate the time point of 100 µM ATP solution supplementation. n = 200. ( E , F ) AUC ( E ) and maximum amplitude ( F ) of the relative intensity from ( D ). ( H ) Ca 2+ influx type classification in Jaw1 KO, Jaw1 IE, and Δcoil cells. Four independent experiments (n = 50). The error bars show ± S.D.; n.s., non-significant; * p < 0.05; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.
    Figure Legend Snippet: Jaw1 increases Ca 2+ influx by changing the Ca 2+ influx pattern. ( A , B , C ) Representative relative intensity traces upon 100 µM ATP stimulation: ( A ) single, ( B ) oscillation, and ( C ) steady reduction types. ( D , G ) Mean ( D ) or five representative ( G ) curves of the relative intensity in Jaw1 KO, Jaw1 IE, and Δcoil IE cells. The closed triangles indicate the time point of 100 µM ATP solution supplementation. n = 200. ( E , F ) AUC ( E ) and maximum amplitude ( F ) of the relative intensity from ( D ). ( H ) Ca 2+ influx type classification in Jaw1 KO, Jaw1 IE, and Δcoil cells. Four independent experiments (n = 50). The error bars show ± S.D.; n.s., non-significant; * p < 0.05; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.

    Techniques Used:

    The Jaw1 augmentative effect on the Ca 2+ influx is maintained even under weak GPCR stimulation. ( A , E ) Five representative relative Fluo-4 fluorescence intensity responses to the stimulation with 1 µM ( A ) or 0.3 µM ( E ) ATP out of 200 cells. The closed triangles show the time point of ATP stimulation. ( B , F ) Ca 2+ influx type classification in each cell line upon stimulation with 1 µM ( B ) or 0.3 µM ( F ) ATP. The ratios were calculated based on the average of four independent wells. ( C , D , G , H ) Graph representing the AUC ( C , G ) and maximum amplitude ( D , H ) of the relative Fluo-4 fluorescence intensity in each cell line upon stimulation with 1 µM ( C , D ) or 0.3 µM ( G , H ) ATP in 200 cells. The error bar shows ± S.D.; **** p < 0.0001. Student’s t -test.
    Figure Legend Snippet: The Jaw1 augmentative effect on the Ca 2+ influx is maintained even under weak GPCR stimulation. ( A , E ) Five representative relative Fluo-4 fluorescence intensity responses to the stimulation with 1 µM ( A ) or 0.3 µM ( E ) ATP out of 200 cells. The closed triangles show the time point of ATP stimulation. ( B , F ) Ca 2+ influx type classification in each cell line upon stimulation with 1 µM ( B ) or 0.3 µM ( F ) ATP. The ratios were calculated based on the average of four independent wells. ( C , D , G , H ) Graph representing the AUC ( C , G ) and maximum amplitude ( D , H ) of the relative Fluo-4 fluorescence intensity in each cell line upon stimulation with 1 µM ( C , D ) or 0.3 µM ( G , H ) ATP in 200 cells. The error bar shows ± S.D.; **** p < 0.0001. Student’s t -test.

    Techniques Used: Fluorescence

    Jaw1 accelerates Ca 2+ efflux from ER . ( A , D ) Mean ( A ) or five representative ( D ) curves of the relative intensity upon 100 µM ATP stimulation under extracellular Ca 2+ free condition in Jaw1 KO and Jaw1 IE cells. n = 200. The closed triangle shows the time point of 100 µM ATP solution supplementation. ( B , C ) AUC ( B ) or maximum amplitude ( C ) of the relative intensity from ( A ). ( E ) Mean curves of the relative intensity upon Ca 2+ depletion in the ER by 2 µM thapsigargin supplementation in 0 mM Ca 2+ and replenishment with 2 mM Ca 2+ . The closed triangle shows the time point of 2 µM thapsigargin solution supplementation. The area surrounded with a black box is enlarged in the right. n = 3. ( F , G ) Maximum amplitude in SOCE ( F ) and time duration to reach the maximum amplitude in the Ca 2+ leakage ( G ) from ( E ). The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; **** p < 0.0001, Student’s t -test.
    Figure Legend Snippet: Jaw1 accelerates Ca 2+ efflux from ER . ( A , D ) Mean ( A ) or five representative ( D ) curves of the relative intensity upon 100 µM ATP stimulation under extracellular Ca 2+ free condition in Jaw1 KO and Jaw1 IE cells. n = 200. The closed triangle shows the time point of 100 µM ATP solution supplementation. ( B , C ) AUC ( B ) or maximum amplitude ( C ) of the relative intensity from ( A ). ( E ) Mean curves of the relative intensity upon Ca 2+ depletion in the ER by 2 µM thapsigargin supplementation in 0 mM Ca 2+ and replenishment with 2 mM Ca 2+ . The closed triangle shows the time point of 2 µM thapsigargin solution supplementation. The area surrounded with a black box is enlarged in the right. n = 3. ( F , G ) Maximum amplitude in SOCE ( F ) and time duration to reach the maximum amplitude in the Ca 2+ leakage ( G ) from ( E ). The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; **** p < 0.0001, Student’s t -test.

    Techniques Used:

    Jaw1 increases the activity of all ITPR subtypes with subtle differences. ( A ) Jaw1 KO, Jaw1 IE, and Δcoil IE cell lysates were subjected to co-immunoprecipitation followed by western blotting. ( B ) Jaw1 KO, R1 SE #11 or #17, R2 SE #5 or #9, and R3 SE #5 or #7 cells were subjected to western blotting. Images used for western blots of ( A ) and ( B ) are shown in Supplementary Fig. , online. ( C , D , F , G , I , J ) Mean ( C , F , I ) or five representative ( D , G , J ) curves of the relative intensity in R1 SE, R2 SE, and R3 SE with or without Jaw1 IE cells. Closed triangles indicate the time point of 100 µM ATP solution supplementation. n = 200. ( E , H , K ) Classification of the Ca 2+ influx type in each cell line. Four independent experiments (n = 50). ( L , M ) AUC ( L ) or maximum amplitude ( M ) of the relative intensity from ( C ), ( F ), and ( I ). The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.
    Figure Legend Snippet: Jaw1 increases the activity of all ITPR subtypes with subtle differences. ( A ) Jaw1 KO, Jaw1 IE, and Δcoil IE cell lysates were subjected to co-immunoprecipitation followed by western blotting. ( B ) Jaw1 KO, R1 SE #11 or #17, R2 SE #5 or #9, and R3 SE #5 or #7 cells were subjected to western blotting. Images used for western blots of ( A ) and ( B ) are shown in Supplementary Fig. , online. ( C , D , F , G , I , J ) Mean ( C , F , I ) or five representative ( D , G , J ) curves of the relative intensity in R1 SE, R2 SE, and R3 SE with or without Jaw1 IE cells. Closed triangles indicate the time point of 100 µM ATP solution supplementation. n = 200. ( E , H , K ) Classification of the Ca 2+ influx type in each cell line. Four independent experiments (n = 50). ( L , M ) AUC ( L ) or maximum amplitude ( M ) of the relative intensity from ( C ), ( F ), and ( I ). The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Techniques Used: Activity Assay, Immunoprecipitation, Western Blot

    A model of the Jaw1 function as a regulator of the ITPRs activity upon GPCR stimulation. ( A ) Jaw1 promotes Ca 2+ release activity of ITPRs and increases Ca 2+ influx into cytoplasm and mitochondria upon GPCR stimulation. Jaw1 changes the Ca 2+ influx pattern from oscillated response to continuous response in high GPCR stimulation, and from weak response to strong response in low GPCR stimulation.
    Figure Legend Snippet: A model of the Jaw1 function as a regulator of the ITPRs activity upon GPCR stimulation. ( A ) Jaw1 promotes Ca 2+ release activity of ITPRs and increases Ca 2+ influx into cytoplasm and mitochondria upon GPCR stimulation. Jaw1 changes the Ca 2+ influx pattern from oscillated response to continuous response in high GPCR stimulation, and from weak response to strong response in low GPCR stimulation.

    Techniques Used: Activity Assay



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    anti-human jaw1 antibody  (Operon Biotech)
    90
    Operon Biotech anti-human jaw1 antibody
    <t>Jaw1</t> increases the Ca 2+ influx depending on its own expression level. ( A , B , E , F , L ) Mean curves of relative Fluo-4 ( A , E , L ) or Rhod-2 ( B , F ) intensity upon 100 µM ATP stimulation measured using a plate reader. WT, Jaw1 KO #7, and Jaw1 KO #17 cells ( A , B ); Jaw1 KO, Jaw1 IE, and Δcoil IE cells ( E , F ); or Jaw1 KO, Jaw1 IE medium, and Jaw1 IE high cells ( L ). The closed triangles indicate the time point of 100 µM ATP solution supplementation. ( C , D , G , H , M ) AUC in ( A ), ( B ), ( E ), ( F ), and ( L ) are shown in ( C ), ( D ), ( G ), ( H ), and ( M ), respectively. n = 3. ( I ) Relative expression level of jaw1 mRNA to gapdh in WT and Jaw1 IE cells measured by RT-qPCR. N = 3. ( J ) Jaw1 KO and Jaw1 IE cells treated with 0.25 or 200 ng/mL of Dox and subjected to western blotting. Images used for western blots are shown in Supplementary Fig. online. ( K ) The relative protein expression level of Jaw1 with α-Tubulin in ( J ). n = 3. The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.
    Anti Human Jaw1 Antibody, supplied by Operon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-human jaw1 antibody/product/Operon Biotech
    Average 90 stars, based on 1 article reviews
    anti-human jaw1 antibody - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    rabbit polyclonal anti-human jaw1 antibody was raised against truncated jaw1 encoding amino acids 1–440  (Operon Biotech)
    90
    Operon Biotech rabbit polyclonal anti-human jaw1 antibody was raised against truncated jaw1 encoding amino acids 1–440
    <t>Jaw1</t> increases the Ca 2+ influx depending on its own expression level. ( A , B , E , F , L ) Mean curves of relative Fluo-4 ( A , E , L ) or Rhod-2 ( B , F ) intensity upon 100 µM ATP stimulation measured using a plate reader. WT, Jaw1 KO #7, and Jaw1 KO #17 cells ( A , B ); Jaw1 KO, Jaw1 IE, and Δcoil IE cells ( E , F ); or Jaw1 KO, Jaw1 IE medium, and Jaw1 IE high cells ( L ). The closed triangles indicate the time point of 100 µM ATP solution supplementation. ( C , D , G , H , M ) AUC in ( A ), ( B ), ( E ), ( F ), and ( L ) are shown in ( C ), ( D ), ( G ), ( H ), and ( M ), respectively. n = 3. ( I ) Relative expression level of jaw1 mRNA to gapdh in WT and Jaw1 IE cells measured by RT-qPCR. N = 3. ( J ) Jaw1 KO and Jaw1 IE cells treated with 0.25 or 200 ng/mL of Dox and subjected to western blotting. Images used for western blots are shown in Supplementary Fig. online. ( K ) The relative protein expression level of Jaw1 with α-Tubulin in ( J ). n = 3. The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.
    Rabbit Polyclonal Anti Human Jaw1 Antibody Was Raised Against Truncated Jaw1 Encoding Amino Acids 1–440, supplied by Operon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti-human jaw1 antibody was raised against truncated jaw1 encoding amino acids 1–440/product/Operon Biotech
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-human jaw1 antibody was raised against truncated jaw1 encoding amino acids 1–440 - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

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    Jaw1 increases the Ca 2+ influx depending on its own expression level. ( A , B , E , F , L ) Mean curves of relative Fluo-4 ( A , E , L ) or Rhod-2 ( B , F ) intensity upon 100 µM ATP stimulation measured using a plate reader. WT, Jaw1 KO #7, and Jaw1 KO #17 cells ( A , B ); Jaw1 KO, Jaw1 IE, and Δcoil IE cells ( E , F ); or Jaw1 KO, Jaw1 IE medium, and Jaw1 IE high cells ( L ). The closed triangles indicate the time point of 100 µM ATP solution supplementation. ( C , D , G , H , M ) AUC in ( A ), ( B ), ( E ), ( F ), and ( L ) are shown in ( C ), ( D ), ( G ), ( H ), and ( M ), respectively. n = 3. ( I ) Relative expression level of jaw1 mRNA to gapdh in WT and Jaw1 IE cells measured by RT-qPCR. N = 3. ( J ) Jaw1 KO and Jaw1 IE cells treated with 0.25 or 200 ng/mL of Dox and subjected to western blotting. Images used for western blots are shown in Supplementary Fig. online. ( K ) The relative protein expression level of Jaw1 with α-Tubulin in ( J ). n = 3. The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.

    Journal: Scientific Reports

    Article Title: Jaw1/LRMP increases Ca 2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype

    doi: 10.1038/s41598-022-13620-4

    Figure Lengend Snippet: Jaw1 increases the Ca 2+ influx depending on its own expression level. ( A , B , E , F , L ) Mean curves of relative Fluo-4 ( A , E , L ) or Rhod-2 ( B , F ) intensity upon 100 µM ATP stimulation measured using a plate reader. WT, Jaw1 KO #7, and Jaw1 KO #17 cells ( A , B ); Jaw1 KO, Jaw1 IE, and Δcoil IE cells ( E , F ); or Jaw1 KO, Jaw1 IE medium, and Jaw1 IE high cells ( L ). The closed triangles indicate the time point of 100 µM ATP solution supplementation. ( C , D , G , H , M ) AUC in ( A ), ( B ), ( E ), ( F ), and ( L ) are shown in ( C ), ( D ), ( G ), ( H ), and ( M ), respectively. n = 3. ( I ) Relative expression level of jaw1 mRNA to gapdh in WT and Jaw1 IE cells measured by RT-qPCR. N = 3. ( J ) Jaw1 KO and Jaw1 IE cells treated with 0.25 or 200 ng/mL of Dox and subjected to western blotting. Images used for western blots are shown in Supplementary Fig. online. ( K ) The relative protein expression level of Jaw1 with α-Tubulin in ( J ). n = 3. The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.

    Article Snippet: Rabbit polyclonal anti-human Jaw1 antibody was raised against truncated Jaw1 encoding amino acids 1–440 (Operon Biotech).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Jaw1 increases Ca 2+ influx by changing the Ca 2+ influx pattern. ( A , B , C ) Representative relative intensity traces upon 100 µM ATP stimulation: ( A ) single, ( B ) oscillation, and ( C ) steady reduction types. ( D , G ) Mean ( D ) or five representative ( G ) curves of the relative intensity in Jaw1 KO, Jaw1 IE, and Δcoil IE cells. The closed triangles indicate the time point of 100 µM ATP solution supplementation. n = 200. ( E , F ) AUC ( E ) and maximum amplitude ( F ) of the relative intensity from ( D ). ( H ) Ca 2+ influx type classification in Jaw1 KO, Jaw1 IE, and Δcoil cells. Four independent experiments (n = 50). The error bars show ± S.D.; n.s., non-significant; * p < 0.05; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.

    Journal: Scientific Reports

    Article Title: Jaw1/LRMP increases Ca 2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype

    doi: 10.1038/s41598-022-13620-4

    Figure Lengend Snippet: Jaw1 increases Ca 2+ influx by changing the Ca 2+ influx pattern. ( A , B , C ) Representative relative intensity traces upon 100 µM ATP stimulation: ( A ) single, ( B ) oscillation, and ( C ) steady reduction types. ( D , G ) Mean ( D ) or five representative ( G ) curves of the relative intensity in Jaw1 KO, Jaw1 IE, and Δcoil IE cells. The closed triangles indicate the time point of 100 µM ATP solution supplementation. n = 200. ( E , F ) AUC ( E ) and maximum amplitude ( F ) of the relative intensity from ( D ). ( H ) Ca 2+ influx type classification in Jaw1 KO, Jaw1 IE, and Δcoil cells. Four independent experiments (n = 50). The error bars show ± S.D.; n.s., non-significant; * p < 0.05; *** p < 0.001; **** p < 0.0001, Tukey–Kramer’s t -test.

    Article Snippet: Rabbit polyclonal anti-human Jaw1 antibody was raised against truncated Jaw1 encoding amino acids 1–440 (Operon Biotech).

    Techniques:

    The Jaw1 augmentative effect on the Ca 2+ influx is maintained even under weak GPCR stimulation. ( A , E ) Five representative relative Fluo-4 fluorescence intensity responses to the stimulation with 1 µM ( A ) or 0.3 µM ( E ) ATP out of 200 cells. The closed triangles show the time point of ATP stimulation. ( B , F ) Ca 2+ influx type classification in each cell line upon stimulation with 1 µM ( B ) or 0.3 µM ( F ) ATP. The ratios were calculated based on the average of four independent wells. ( C , D , G , H ) Graph representing the AUC ( C , G ) and maximum amplitude ( D , H ) of the relative Fluo-4 fluorescence intensity in each cell line upon stimulation with 1 µM ( C , D ) or 0.3 µM ( G , H ) ATP in 200 cells. The error bar shows ± S.D.; **** p < 0.0001. Student’s t -test.

    Journal: Scientific Reports

    Article Title: Jaw1/LRMP increases Ca 2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype

    doi: 10.1038/s41598-022-13620-4

    Figure Lengend Snippet: The Jaw1 augmentative effect on the Ca 2+ influx is maintained even under weak GPCR stimulation. ( A , E ) Five representative relative Fluo-4 fluorescence intensity responses to the stimulation with 1 µM ( A ) or 0.3 µM ( E ) ATP out of 200 cells. The closed triangles show the time point of ATP stimulation. ( B , F ) Ca 2+ influx type classification in each cell line upon stimulation with 1 µM ( B ) or 0.3 µM ( F ) ATP. The ratios were calculated based on the average of four independent wells. ( C , D , G , H ) Graph representing the AUC ( C , G ) and maximum amplitude ( D , H ) of the relative Fluo-4 fluorescence intensity in each cell line upon stimulation with 1 µM ( C , D ) or 0.3 µM ( G , H ) ATP in 200 cells. The error bar shows ± S.D.; **** p < 0.0001. Student’s t -test.

    Article Snippet: Rabbit polyclonal anti-human Jaw1 antibody was raised against truncated Jaw1 encoding amino acids 1–440 (Operon Biotech).

    Techniques: Fluorescence

    Jaw1 accelerates Ca 2+ efflux from ER . ( A , D ) Mean ( A ) or five representative ( D ) curves of the relative intensity upon 100 µM ATP stimulation under extracellular Ca 2+ free condition in Jaw1 KO and Jaw1 IE cells. n = 200. The closed triangle shows the time point of 100 µM ATP solution supplementation. ( B , C ) AUC ( B ) or maximum amplitude ( C ) of the relative intensity from ( A ). ( E ) Mean curves of the relative intensity upon Ca 2+ depletion in the ER by 2 µM thapsigargin supplementation in 0 mM Ca 2+ and replenishment with 2 mM Ca 2+ . The closed triangle shows the time point of 2 µM thapsigargin solution supplementation. The area surrounded with a black box is enlarged in the right. n = 3. ( F , G ) Maximum amplitude in SOCE ( F ) and time duration to reach the maximum amplitude in the Ca 2+ leakage ( G ) from ( E ). The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; **** p < 0.0001, Student’s t -test.

    Journal: Scientific Reports

    Article Title: Jaw1/LRMP increases Ca 2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype

    doi: 10.1038/s41598-022-13620-4

    Figure Lengend Snippet: Jaw1 accelerates Ca 2+ efflux from ER . ( A , D ) Mean ( A ) or five representative ( D ) curves of the relative intensity upon 100 µM ATP stimulation under extracellular Ca 2+ free condition in Jaw1 KO and Jaw1 IE cells. n = 200. The closed triangle shows the time point of 100 µM ATP solution supplementation. ( B , C ) AUC ( B ) or maximum amplitude ( C ) of the relative intensity from ( A ). ( E ) Mean curves of the relative intensity upon Ca 2+ depletion in the ER by 2 µM thapsigargin supplementation in 0 mM Ca 2+ and replenishment with 2 mM Ca 2+ . The closed triangle shows the time point of 2 µM thapsigargin solution supplementation. The area surrounded with a black box is enlarged in the right. n = 3. ( F , G ) Maximum amplitude in SOCE ( F ) and time duration to reach the maximum amplitude in the Ca 2+ leakage ( G ) from ( E ). The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; **** p < 0.0001, Student’s t -test.

    Article Snippet: Rabbit polyclonal anti-human Jaw1 antibody was raised against truncated Jaw1 encoding amino acids 1–440 (Operon Biotech).

    Techniques:

    Jaw1 increases the activity of all ITPR subtypes with subtle differences. ( A ) Jaw1 KO, Jaw1 IE, and Δcoil IE cell lysates were subjected to co-immunoprecipitation followed by western blotting. ( B ) Jaw1 KO, R1 SE #11 or #17, R2 SE #5 or #9, and R3 SE #5 or #7 cells were subjected to western blotting. Images used for western blots of ( A ) and ( B ) are shown in Supplementary Fig. , online. ( C , D , F , G , I , J ) Mean ( C , F , I ) or five representative ( D , G , J ) curves of the relative intensity in R1 SE, R2 SE, and R3 SE with or without Jaw1 IE cells. Closed triangles indicate the time point of 100 µM ATP solution supplementation. n = 200. ( E , H , K ) Classification of the Ca 2+ influx type in each cell line. Four independent experiments (n = 50). ( L , M ) AUC ( L ) or maximum amplitude ( M ) of the relative intensity from ( C ), ( F ), and ( I ). The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Scientific Reports

    Article Title: Jaw1/LRMP increases Ca 2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype

    doi: 10.1038/s41598-022-13620-4

    Figure Lengend Snippet: Jaw1 increases the activity of all ITPR subtypes with subtle differences. ( A ) Jaw1 KO, Jaw1 IE, and Δcoil IE cell lysates were subjected to co-immunoprecipitation followed by western blotting. ( B ) Jaw1 KO, R1 SE #11 or #17, R2 SE #5 or #9, and R3 SE #5 or #7 cells were subjected to western blotting. Images used for western blots of ( A ) and ( B ) are shown in Supplementary Fig. , online. ( C , D , F , G , I , J ) Mean ( C , F , I ) or five representative ( D , G , J ) curves of the relative intensity in R1 SE, R2 SE, and R3 SE with or without Jaw1 IE cells. Closed triangles indicate the time point of 100 µM ATP solution supplementation. n = 200. ( E , H , K ) Classification of the Ca 2+ influx type in each cell line. Four independent experiments (n = 50). ( L , M ) AUC ( L ) or maximum amplitude ( M ) of the relative intensity from ( C ), ( F ), and ( I ). The error bar shows ± S.D.; n.s., non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: Rabbit polyclonal anti-human Jaw1 antibody was raised against truncated Jaw1 encoding amino acids 1–440 (Operon Biotech).

    Techniques: Activity Assay, Immunoprecipitation, Western Blot

    A model of the Jaw1 function as a regulator of the ITPRs activity upon GPCR stimulation. ( A ) Jaw1 promotes Ca 2+ release activity of ITPRs and increases Ca 2+ influx into cytoplasm and mitochondria upon GPCR stimulation. Jaw1 changes the Ca 2+ influx pattern from oscillated response to continuous response in high GPCR stimulation, and from weak response to strong response in low GPCR stimulation.

    Journal: Scientific Reports

    Article Title: Jaw1/LRMP increases Ca 2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype

    doi: 10.1038/s41598-022-13620-4

    Figure Lengend Snippet: A model of the Jaw1 function as a regulator of the ITPRs activity upon GPCR stimulation. ( A ) Jaw1 promotes Ca 2+ release activity of ITPRs and increases Ca 2+ influx into cytoplasm and mitochondria upon GPCR stimulation. Jaw1 changes the Ca 2+ influx pattern from oscillated response to continuous response in high GPCR stimulation, and from weak response to strong response in low GPCR stimulation.

    Article Snippet: Rabbit polyclonal anti-human Jaw1 antibody was raised against truncated Jaw1 encoding amino acids 1–440 (Operon Biotech).

    Techniques: Activity Assay